A process for the preparation of oligoribonucleotides of definend predetermined sequence containing the natural 3'-5'-internucleotide linkage, which comprises reacting an oligonucleotide with a monofunctional substrate in the presence of polynucleotide phosphorylase, to form a product which is longer, by one monomeric unit than the initial oligonucleotide, isolating the product from the reaction mixture, removing the blocking agent and repeating the process a desired number of times, adding by e...