The present disclosure provides methods and DNA molecules for the synthesis of heterologous proteins in the fungus Aureobasidium pullulans either intracellularly or with secretion out of the cells using a regulated xylanase promoter and for secreted protein synthesis, a signal sequence. Further described are kits containing host cells for recombinant protein production, a vector containing an XynA transcription regulatory sequence, and instructions for using the vector to transform the host cell...

An .alpha.-L-arabinofuranosidase enzyme which is highly thermostable, and is effective for the hydrolysis of arabinofuranosyl residues from L-arabinose containing polysaccharides and hemicelluloses is disclosed. The enzyme is produced by color variant Aureobasidium pullulans strain NRRL Y-21792. This .alpha.-L-arabinofuranosidase may be used in conjunction with xylanolytic enzymes for the treatment of hemicellulosic materials to produce fermentable sugars, particularly xylose and L-arabinose.

An antibiotic R106 represented by the general formula I): ##STR1## wherein: R is methyl or ethyl; X.sub.1 is MePhe, .beta.-HOMePhe or Phe; X.sub.2 is allo-Ile, Val or Leu; X.sub.3 is MeVal or Val; X.sub.4 is .beta.-HOMeVal, .gamma.-HOMeVal, MeVal, Val, N,.beta.-MeAsp, .beta.-HOMePhe, MePhe, MeDH.sub.2,3 Val or MeDH.sub.3,4 Val is produced by a process which comprises culturing a strain of the genus Aureobasidium that is capable of producing the said antibiotic R106 and collecting the said antibi...

A xylanase from Aureobasidium pullulans having a high specific activity is provided as well as a signal protein for controlling excretion into cell culture medium of proteins to which it is attached. DNA encoding these proteins is also provided.

The invention provides isolated nucleic acid compounds encoding the multiple drug resistance protein of Aureobasidium pullulans. Vectors and transformed host cells comprising the multiple drug resistance-encoding DNA of Aureobasidium pullulans are also provided. The invention further provides assays which utilize these transformed host cells.

An antibiotic R106 represented by the general formula (I): ##STR1## wherein: R is methyl or ethyl; X.sub.1 is MePhe, .beta.-HOMePhe or Phe; X.sub.2 is allo-Ile, Val or Leu; X.sub.3 is MeVal or Val; X.sub.4 is .beta.-HOMeVal, .gamma.HOMeVal, MeVal, Val, N,.beta.-MeAsp, .beta.-HOMePhe, MePhe, MeDH.sub.2,3 Val or MeDH.sub.3,4 Val is produced by a process which comprises culturing a strain of the genus Aureobasidium that is capable of producing the said antibiotic R106 and collecting the said antibi...

The invention relates to Aureobasidium pullulans strains which produce pullulan but virtually no melanin. A process for the preparation of these strains, and a preferred use, are also disclosed.

Strains of Aureobasidium pullulans (de bary) Arnaud can be used for the commercial production of gluconic acid by fermentation in aqueous liquid containing sugar, which in continuous culture from glucose form greater than or equal to 90% and greater than or equal to 90% molar selectivity. The process is conducted with an Fe and Mn optimized medium with a nitrogen-independent (N-independent) ion concentration. The iron (Fe) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to 0.5 mM, ...