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This invention relates to a novel vector constructed by inserting an exogenous RNA fragment into a vector comprising a satellite RNA of a plant virus in a way wherein the replication ability of the satellite is retained as much as possible to convert it to a chimeric RNA molecule. Those novel vectors satisfactorily have conditions necessary as a vector and may effectively be used for transformation of plants, synthesis of proteins and the like.
Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A) a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition func...
A two stage approach is used in which rotation angles are specifically seted for easy mechanization. First stage provides rotations in even 30.degree. increments so that multiplications are replaced by simple shift or shift/add operations. Rotation angles available in the second stage each have sines with at most two nonzero bits in their binary representations.
A cloning vector useful in recombining DNA (i.e. recombinant DNA studies) is made by mutagenesis of a satellite bacteriophage P4 wt or P4 vir.sub.1 and subsequent separation and purification of the mutant progeny in a cesium chloride equilibrium density gradient. On such a cesium chloride gradient the mutant is found in a range of densities from 1.35 to 1.42 g/ml at 24.degree. C. with peaks at about 1.42, 1.39, and 1.35 g/ml.
A high speed vector generating electrical system is provided which provides the deflection voltages required to draw a vector line from one point to another on the screen of a cathode-ray tube. The system is particularly useful for drawing images in any desired pattern on the screen of the cathode-ray tube, whereby the images are composed of a multitude of vector lines. The system of the invention is simplified as compared with the prior art systems of the same general type, and it requires less...
A vector generator provides a linear interpolation between points of a step function or dot waveform wherein the linear interpolation represents the vector sum of the time and amplitude components of the waveform. The vector generator comprises a sampling circuit, an integrator, and a feedback loop. Samples are taken at the appropriate times, setting up a differential current in the system. The differential current is integrated, producing a substantially linear voltage change from the first sam...
In response to the execution of a single loading instruction, the front half and the rear half of a designated vector may be stored in respective vector registers in a single processor operation. For this purpose, a data distribution circuit is interposed between a group of vector registers and a vector data storage for feeding the vector data read out from the storage to a first vector processor designated by an instruction without shifting and for shifting the respective components of the read...
In a method and a vector scope for measuring distortion of a subcarrier signal causing color aberration of a color television signal, a carrier chrominance signal resulting from a color bar signal transmitted in a transmission system and a reference carrier chrominance signal including a signal determining levels of the amplitude and phase distortions of said carrier chrominance signal are demodulated through the same demodulating device and color difference signals thus obtained are displayed i...
There are provided herewith vector precursors and vectors based on Pst I cleaved left and right border fragments of the T region of the Ti plasmid Agrobacterium tumefaciens strain of C58 interligated and ligated into the Pst I site of plasmid pBR322. Antibiotic resistance gene sequence is used to establish vector expression and presence in host cells.
Complementary ramp waves are generated which extend between first and second direct-current levels. During successive time intervals, multiple digit binary words are generated defining the horizontal and vertical components of the vector being generated. Registers store the binary words, and gates respond to the stored digits for controlling a plurality of switches which select for each digit one of the complementary ramp waves or the direct-current levels, depending on whether or not a digit ha...
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