Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.

Boron compound complexing reagents, intermediate reagents of those reagents and methods of synthesizing these reagents are disclosed. These reagents, including those shown as General Formula I and General Formula II may be used, after further reactions described herein, to complex with boronic compounds, such as phenylboronic acid or derivatives thereof. ##STR1##

This invention relates to novel chiral boronic acid compounds which are capable of binding to polyols, particularly to saccharides and are useful in the analysis or separation of diols.

The present invention relates to labelling agents comprising non-proteinaceous boronic acid conjugates having absorption maxima at not less than 600 nm, said label being provided by e.g., an azine, triphenylmethane, cyanine or phthalocyanine dye. The labelling agents are useful in the estimation and quantification of cisdiols such as glycosylated haemoglobin, by virtue of the substantially total absence of overlap with the absorption spectrum of haemoglobin. Oxazine and thiazine dyes exhibiting ...

Boronate complexes are rearranged in the presence of a Lewis acid catalyst to boronic esters, particularly .alpha.-haloboronic esters.

Soluble serine-hydrolytic enzymes are purified by passing a solution at pH 7.5 through a column packed with a granular support to which is covalently bonded a boronic acid to reversibly complex the enzyme; washing the column with a first buffer solution; freeing the enzyme by washing the column with a second buffer solution; and isolating the freed enzyme.

The invention relates to a process for the preparation of organic boronic acid derivatives involving the reaction of an organic compound with diboronic acid in the presence of a Group 8-11 metal catalyst.

Intermediates for the preparation of Peptides comprising C-terminal boronic acid derivatives of lysine, ornithine, and arginine, homoarginine and corresponding isothiouronium analogs thereof, are reversible inhibitors of trypsin-like serine proteases such as thrombin, plasma kallikrein and plasmin.

Boronic acid adducts of radioactive isotopes of rhenium dioxime complexes are useful for labeling biologically active compounds as agents for radiotherapy. These boronic acid adducts of radioactive isotopes of rhenium dioxime complexes have the formula I. ReX(Y).sub.3 Z, wherein X is an anion; Y is a vicinal dioxime having the formula II. ##STR1## or a pharmaceutically acceptable salt thereof, and R.sub.1 and R.sub.2 are each independently hydrogen, halogen, alkyl, aryl, amino or a 5 or 6-member...

Boronic acid adducts of technetium-99m dioxime complexes are useful for imaging the myocardium, hepatobiliary system, brain and blood pool in humans and other mammalian species.