Disclosed is a technique for accelerating the Hantzsch reaction, for the clinical determination of triglyceride, so as to permit the reaction to be analytically complete within 15 minutes at 37.degree. C. As is known, in this Hantzsch reaction, one mole of aldehyde is reacted in a solution predominantly containing alcohol and water with two moles of a betadicarbonyl compound in the presence of ammonia to yield, as a colored derivative, a lutidine. In the disclosed technique, the solution also co...

A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligosaccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by .alpha. and/or .beta.-glucosidase and contain endo-.alpha.-1,4 linkages which are required for amylase activity. Cleavage of the .alpha.-1,4 bonds by amylase produces sm...

A process for the quantitative determination of the transcobalamins TC-I, TC-II, and TC-III in serum. The process comprises incubating the serum with radiolabeled vitamin B-12, passing the resulting mixture through adsorption means, such as a charged cellulose filter or equivalent mini-column, for TC-II at a pH of about 8.5, adsorbing the remaining TC-I and TC-II components on a DEAE-cellulose type adsorbent, selectively desorbing the TC-III component with monopotassium phosphate solution of abo...

Method and apparatus for determining pitch by establishing the fundamental period of an incoming acoustic wave and comparing this fundamental period with the period of certain standard pre-established tones. The name of the standard tone with period closest to the measured fundamental period of the incoming wave is indicated and displayed. Also displayed is an indication of the degree and direction of any deviation between the pitch of the incoming wave and that of the displayed standard tone.

A method and apparatus for determining the relative positions of a first object (1) and a second object (3). The method comprises illuminating the first object (1) so as to produce a shadow (17) of the first object on a surface (15) lying at a known position with respect to the second object (3) and forming a two dimensional image of a scene including the surface (15). The distance between the position of the shadow (17) and a reference position (19) in the two dimensional image is monitored, an...

An improved azobilirubin determination procedure utilizes a stable, dry lyophilized reagent. Dissolving the dry reagent in dilute hydrochloric acid produces a diazo reagent solution which is stable for several days. Cysteine hydrochloride may be added to the assay mixture to destroy excess diazo reagent, stabilize the final color, and remove interfering metal ions.

Disclosed is an optical measurement scale 14 complementary scale reader 10 for determining displacement between the scale and reader. The scale 14 and reader 10 are adapted for use with a liquid C which provides optical communication between the scale 14 and the reader 10. The liquid C is preferably machine coolant or oil. The scale is preferably a stainless steel strip having periodic markings thereon. The scale reader has a window 16 which may have a hard coating (30 FIG. 4) thereon, e.g. diam...

Haplotypes for phased genotypic information are determined from unphased genotypic information. One exemplary method includes separating a dataset that includes genotypic information for a plurality of markers for each of a plurality of individuals into subdatasets, each subdataset including the genotypic information for a subset of the plurality of markers; for each subdataset, identifying haplotypes compatible with marker information for each marker of the subset and for each individual of the...

A process and test kit for the quantitative determination of the individual and total unsaturated Vitamin B-12 binding capacities of the three transcobalamins TC-I, TC-II, and TC-III in serum. The process comprises incubating a serum sample with a solution of .sup.57 Co Vitamin B-12, contacting the resulting mixture with a TC-II adsorbent and with a DEAE-cellulose adsorbent for TC-I and TC-III, measuring the total radioactivity adsorbed to said adsorbents, selectively desorbing TC-III from the D...

Colorimetric urea determination method and reagent in which urea in a liquid sample reacts with o-phthalaldehyde and chromotropic acid or one of its salts to produce an intensely colored reaction product whose concentration is linearly related to the concentration of urea in the sample and which follows Beer's law over a wide range of urea concentrations.