
There is disclosed a method of determining the toxicity of a fluid sample comprising the steps of: (i) mixing the sample with a suspension of light emitting organisms; (ii) monitoring the light output (E) of the mixture continually over a period of time (t.sub.o -t.sub.n) using a photodetector device sensitive to the wave length of the emitted light; and (iii) determining the differential d(log E)/dt to give a measure of toxicity concentration.











