Glucose isomerase is immobilized in an active form by adsorbing the glucose isomerase onto a colloidal silica. The enzyme is contacted with the colloidal silica and the resulting composite solidified by freezing. Optionally, the composite may be gelatinized prior to freezing. The composite is then used for the isomerization of glucose to fructose.

The present invention relates to a glucose sensor (2) including an electrode (32) which includes a conductive component and glucose dehydrogenase immobilized to the conductive component. As the glucose dehydrogenase, use is made of a protein complex including a catalytic activity subunit which has glucose dehydrogenase activity, and an electron mediator subunit for supplying an electron donated from the catalytic activity subunit to the conductive component. Preferably, the glucose sensor (2) is...

A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residu...

The present invention is directed to a reagent composition for use in detection and quantification of glucose in urine comprising a first chromogenic indicator reagent combination capable of color development to indicate the presence of a lower concentration of glucose, a second chromogenic indicator reagent combination capable of color development to indicate the presence of a higher concentration of glucose, wherein the first chromogenic indicator prevents color development of the second chrom...

A glucose sensor package system that includes a glucose sensor and a protective package that indicates exposure to temperature changes to indicate proper temperature control. Also covered are methods of transporting and sterilizing the package. In addition, glucose sensors directed to various sizing and positioning of the electrodes on the glucose sensor are covered.

A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow "one-point" in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentr...

For sakes of eliminating displacement zone and fully utilizing the void volume in traditional chromatography, a separation process herein disclosed is for better efficiency in separation of mixed solution of glucose and fructose into glucose and fructose solution. More specifically, the process implements a new mass transfer method onto an alkaline-earth metal cation exchanger bed for proceeding like SMB process, yet, in a single bed or multiple beds in a bundle with batch operation mode. Said n...

Provided are, among other things, lipid-lowering or glucose-lowering methods and compounds, particularly lipid-lowering or glucose-lowering compounds, with an A-B ring structure as follows: ##STR1## wherein the dotted line between carbons 5 and 6, the dotted line between carbon 4 and R.sup.2, and the dotted line between carbon 11 and R.sup.3 each independently represent an optional double bond from the respective numbered carbon to a linked carbon, and wherein X is hydroxy or, together with Y is...

The present invention relates to recombinant-DNA-technology, and particularly to genes involved in the control of basic metabolic processes in fungi. The invention specifically provides a mutated form of the native glucose repressor gene cre of filamentous fungi, wherein the mutation is situated in the C-terminal domain, the N-terminal first zinc finger being intact and the C-terminal region including the second zinc finger being mutated so that the viability of a strain carrying said mutated ge...

A glucose sensor package system that includes a glucose sensor and a protective package that indicates exposure to temperature changes to indicate proper temperature control. Also covered are methods of transporting and sterilizing the package. In addition, glucose sensors directed to various sizing and positioning of the electrodes on the glucose sensor are covered.