A method for the simultaneous determination of glucose and urea in a specimen with a single reagent system. A reagent system containing a reactant for each of glucose and urea to be determined is added to a specimen. The reagent system is reacted with the specimen such that each of glucose and urea react with their respective reactant simultaneously. Each reactant is selected such that it is capable of giving an absorbance band for glucose and urea which permits their simultaneous determination....

New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.

A sample of oral fluid is collected by an absorbent nonreactive collecting swab brought into contact with a favorable surface in the oral cavity. The fluid is interstitial transudate selectively collected from the vestibule region of the oral cavity at the junction of the superior labial mucous membrane and the superior gingivae between the upper canine teeth. The collecting swab is an open cell foam material with a fine (e.g., 0.3 mm diameter) cell size. The collecting swab is placed in a cylin...

A glucose monitor, and related method, determines the concentration of glucose in a sample by monitoring fluorescent light produced directly by any glucose present in the sample. The glucose monitor illuminates the sample with ultraviolet excitation light that induces any glucose present in the sample to fluoresce, with the fluorescent light being monitored and processed to determine the concentration of glucose in the sample. A sensor monitors the return light, which includes fluorescent light ...

The invention provides a nonenzymatic method useful for the semiquantitative determination of glucose, a test composition and test device. Glucose concentration in an aqueous test sample can be determined by preparing a test solution by contacting an aqueous test sample and a dihydroxide component, at an initial pH above 6.5, capable of forming a complex with glucose which complex formation releases a proton, and determining the final pH of the test solution. The invention also provides a self-i...

The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst.

A process to produce solid glucose from a hydrolyzate consisting of a mixture of glucose, water, and an acid used in the hydrolysis of a biomass material is covered herein. In the process, the hydrolyzate is concentrated, as required, to form two phases: a solid glucose phase and an acidic liquid phase. The phases are formed in a vessel where they are separated for recovery of the acidic liquid phase. The solid glucose phase, containing residual acidic liquid phase, is then extracted to remove m...

Glucose isomerase bound to an inert carrier is maintained in suspension while being contacted with a glucose containing solution under specific conditions whereby a portion of the glucose is converted to fructose.

There is provided an enzymatic process for interconverting aqueous solutions of dextrose and fructose with newly discovered glucose isomerases. The glucose isomerases are obtained from cultures of Flavobacterium devorans NRRL B-5384, Flavorbacterium devorans ATCC 10829, Brevibacterium incertum NRRL B-5383 and Streptomyces phaeochromogenes ATCC 15486.

Glucose contained in urine is determined by contacting a sample with a diagnostic composition comprising an absorbent carrier impregnated with glucose oxidase, peroxidase, an indicator compound such as o-tolidine, and as a buffer, 2-(N-morpholino)-ethane-sulfonic acid.