The present invention discloses an assay for nucleic acid which comprises the steps of; (a) providing a probe material comprising; (i) a sequence of nucleic acids complementary to a given target sequence and, (ii) a first ligand chemically linked thereto and capable of a specific binding reaction with an antiligand; (b) contacting the said probe material with an assay system comprising: (i) a suitable mediator, enzyme, substrate system capable of transferring charge to an electrode surface when ...

The crosslinking agent contains an acylhydrazide moiety, through which it is linked to a cytosine residue on the first chain; and an .alpha.-haloketal moiety which, after attachment of the crosslinking agent to the first chain, is hydrolyzed to an .alpha.-haloketone moiety, through which linking is effected to the base-paired guanine residue on the second chain.

An improved method and device for nucleic acid hybridization assay employing combined direct an alternating field electrophoresis are disclosed. In the method of the present invention, a sample is hybridized with nucleic acid probe and is contacted with a support medium where direct and alternating electric fields are applied. Under the influence of the electric fields, hybrid separates from non-specifically bound nucleic acid probe. The hybrid may be measured on the support medium itself as on ...

The invention relates to a mechanical support module for the construction of different models of nucleic acids or the like. A module of this type consists of at least one frame (110) carrying a plurality of (n) plates (255) intended for carrying balls representing atoms and sticks or the like representing bonds, arranged so that each plate (255) contains four bases. The arrangement of the bases on the plates and of the plates in space is such that the pathway (T) followed by the genetic informat...

Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling re...

Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling re...

A method for carrying out a nucleic acid hybridization test to detect a target nucleic acid sequence, the method including the steps of (1) providing a polymer molecule bonded to a first single-stranded nucleic acid sequence which is either (a) a first probe capable of hybridizing to the target nucleic acid sequence, or (b) a second nucleic acid sequence capable of hybridizing to a third nucleic acid sequence bonded to a first probe capable of hybridizing to the target nucleic acid sequence; pro...

A method of extracting nucleic acids from a crude buffy coat fraction in an aqueous medium includes the steps of treating the buffy coat fraction to break open the white blood cells to release the proteins and the nucleic acids in the cells into the aqueous medium; solubilizing the proteins and the nucleic acids in the aqueous medium; and precipitating the nucleic acids under conditions under which the proteins remain in solution.

A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution con...

The process for separating the proteinaceous materials from nucleic acids involves contacting a solution containing the proteinaceous materials and nucleic acids with a solid phase extraction material capable of binding proteins to form bound and unbound fractions and then isolating the unbound fraction containing the nucleic acids.